FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005734796

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005734796
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18835240
Sequences flagged as poor quality0
Sequence length75
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAGACG62700.011.1699431
AGGGGGG46750.09.5936571
CGGGGTT49900.09.401786
ACGGGGT50800.09.1670645
ACCACGC49400.09.14839512
AGGGGAC61250.08.899661
AGGCGCC33650.08.7147781
ACGCCCG17450.08.50129615
GACGGGG54150.08.4722654
GAGACGG74200.08.4614312
AGGGCCG20000.08.4525661
AGGCGCG14750.08.42040351
AGACGGG60400.08.3952513
AGGGGAG125000.08.2524641
TGACGAC8851.2623786E-98.1865041
GACCGAT5907.9418605E-68.1856352
CCACGCC55850.08.15372313
GGCGTAC4250.00132358288.1164173
AGGGTAC40150.08.0772731
AGGGGTG88300.08.048761
Produced by FastQC (version 0.11.5)