FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005734797

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005734797
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31558867
Sequences flagged as poor quality0
Sequence length75
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAGACG130250.011.31308651
AGAGCGA78150.010.0678521
GAGACGG158900.08.9451282
AGGGCGC52000.08.8926751
CGGGGTT112350.08.87461956
AGACGGG128350.08.8701023
ACGGGGT110000.08.8446095
AGAGCGG45550.08.409411
GTCTCGA134050.08.26145133
GACGGGG124950.08.1176024
AGAGCCG52300.08.0498741
AGGGCGG80600.08.0492191
TCGAACT99600.07.93267636
CTCGAAC99850.07.774266235
CGAACTC98500.07.706053737
AGGGAGG379600.07.56359241
GAGCGAG81750.07.51182652
AGGAGGC375750.07.50332981
AGTCCCA265650.07.4824461
CTACTCG77900.07.43996059
Produced by FastQC (version 0.11.5)