FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005734799

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005734799
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25755885
Sequences flagged as poor quality0
Sequence length75
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAGACG82250.010.0276481
CCGGCTA43950.08.79088519
CGTTTCG7555.344191E-98.682133
TAGGCCG15600.08.6273431
GAGACGG97700.08.5455632
ACGCCCG21900.08.50608815
CGGCTAA43400.08.50493320
ACGGGGT70850.08.326685
AGAGCGA48700.08.2907521
CGGGGTT72950.08.228896
AGTCCCG17200.08.2260711
AGGAGCG23600.08.04243851
AGGGCCG24100.08.0187761
AGGGACG23700.08.0085041
AGACGGG82600.07.97747763
GACGGGG77550.07.91863734
TCGAGCG7001.1409575E-67.88588148
GCGCTTT36100.07.836821652
AGACCCG15450.07.81765751
CGTTGGA64350.07.77426867
Produced by FastQC (version 0.11.5)