FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005734800

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005734800
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25755885
Sequences flagged as poor quality0
Sequence length75
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAGACG86100.011.1398491
AGTCGCG4301.4529067E-59.6283191
AGGAGCG23450.09.1219251
AGGGGCG28950.09.0573831
AGGGCGG33250.08.923691
AGAGCGA50350.08.9080271
AGGGGAT105900.08.6009351
AGGCGCC45550.08.55909251
AGGGGGC85400.08.403171
GAGACGG101400.08.4031122
AGGGGAA217600.08.3558261
AGGGCCG26650.08.2855321
GACGGGG76350.08.2676944
AGGGGCC92550.08.20132351
AGACGGG84300.08.1837353
CGGCTAA43100.08.16473420
AGGGGAC87550.08.1574021
AGGGGGG65950.08.1087621
AGGGATG153500.08.0915521
ACGGGGT74300.08.03177455
Produced by FastQC (version 0.11.5)