FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005734807

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005734807
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38837741
Sequences flagged as poor quality0
Sequence length75
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG17050.011.33195446
AGAGACG128150.011.0406681
CGTATGC19150.010.44981844
TCGTATG23950.09.65184743
AGAGCGA80500.09.5595861
CTCGTAT21400.09.35112342
CGGGGTT105200.09.2153456
ACGGGGT104850.09.1801915
GCGCTTT60650.09.15857352
TCGAACT94000.09.02927536
GTCTCGA132750.09.01821133
CTCGAAC95550.08.91853335
CGAACTC91350.08.80026637
GAGACGG149650.08.760132
GACGGGG111450.08.6054914
AGACGGG122100.08.5613
AGACGCG10551.8189894E-128.5045421
GAGCGAG76650.08.2364982
GCGAGAC64950.08.2331034
GGTCTCG141150.08.09039732
Produced by FastQC (version 0.11.5)