FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005734808

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005734808
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38837741
Sequences flagged as poor quality0
Sequence length75
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAGACG133550.012.5809161
ACGGGGT111300.010.2273915
CGGGGTT110250.010.1059416
AGAGCGA83050.010.0947091
GAGACGG152600.09.9690852
GACGGGG119850.09.7853754
AGACGGG128200.09.6324343
AGGGGCG46900.09.1217051
AGGGACG39950.09.0677541
GTCTCGA137850.09.03455733
TCGAACT100800.08.89902136
CTCGAAC99100.08.87756235
CGAACTC96150.08.86292537
CTCGATC55200.08.56265935
GAGCGAG74750.08.3990752
GCGAGAC64700.08.31681254
GGTCTCG147750.08.17238332
GCGCTTT62550.07.943607352
TCTCGAA114850.07.90019734
AGGGGAG260250.07.8744981
Produced by FastQC (version 0.11.5)