FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005734809

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005734809
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences83246552
Sequences flagged as poor quality0
Sequence length126
%GC47

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA1048600.052.0899281
TCAACGC1134700.047.1800234
CAACGCA1163500.046.0257955
ATCAACG1166300.045.9840243
GGTATCA566850.044.017471
AACGCAG1253350.042.8363466
TATCAAC1346800.040.1335452
ACGCAGA1341850.039.9928137
CGCAGAG1484150.036.1340568
TCGTATC159600.033.34799120
TGCCGTT248200.031.81508120
AGTACGG974900.030.7506912-13
GCAGAGT1778700.030.3425679
AGAGTAC1495900.029.7622310-11
TACGGGG791400.028.24608614-15
GTACGGG1016550.026.4842214-15
GAGTACG1021400.026.39922312-13
CTCCGAC375100.026.362648120
TCTCGTT326800.026.292984120
CGAGACC450350.023.889608120
Produced by FastQC (version 0.11.5)