FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005734810

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005734810
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences83246552
Sequences flagged as poor quality0
Sequence length126
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC827850.046.9532554
GTATCAA886550.046.498451
CAACGCA857350.045.3738865
ATCAACG867400.044.81073
AACGCAG923300.042.2519846
ACGCAGA957000.040.715357
TATCAAC1060200.037.5714952
CGCAGAG1102500.035.323028
GGTATCA517000.035.134681
AGTACGG992750.030.83938612-13
AGAGTAC1098150.028.45551310-11
TACGGGG798350.028.29299514-15
GCAGAGT1391350.028.1851129
GTACGGG1030400.027.10890414-15
GAGTACG1036650.027.02934612-13
CAGAGTA1266150.022.43301610-11
ACGGGGG435550.019.38310816-17
GTATATA418750.017.3656271
GTATTAG300200.017.022371
CGCCGTT132200.016.927406120
Produced by FastQC (version 0.11.5)