FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005734811

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005734811
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences83730960
Sequences flagged as poor quality0
Sequence length126
%GC47

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA1107050.055.0646321
TCAACGC1248750.047.4590454
CAACGCA1284050.046.1715165
ATCAACG1292450.045.9240073
GGTATCA586650.045.0945471
AACGCAG1366650.043.4335256
ACGGACC263500.041.170918120
TATCAAC1464900.040.6657942
ACGCAGA1463650.040.5095947
CGCAGAG1601700.037.00318
TCGTATC157200.036.452183120
TGCCGTT251700.033.7323120
AGAGTAC1607850.031.55938110-11
AGTACGG1063050.031.50229812-13
GCAGAGT1931100.030.862129
TACGGGG887700.029.41933314-15
TCTCGTT350000.028.115675120
GTACGGG1095350.026.47945214-15
GAGTACG1137300.025.53941712-13
CTCCGAC387250.025.240757120
Produced by FastQC (version 0.11.5)