FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005734817

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005734817
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28974539
Sequences flagged as poor quality0
Sequence length75
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAGCGA74950.09.7149151
AGAGACG125950.09.1237691
AGGGCGG55250.08.6193591
AGAGCGG33400.08.265551
GAGCGAG70050.08.1754422
AGTCCCG29000.08.0916881
TAGGCGC14500.08.0916881
TAGGCCG21250.07.9572921
AGGACCG23200.07.88344571
AGGAGCG40900.07.8467141
AGTCCCA265850.07.76234051
AGGGCCG48250.07.65271
AGGCGCC82550.07.48279951
GCGAGAC60750.07.43939734
AGGACGA28000.07.3947141
AGGGATC102100.07.36815831
GAGACGG154200.07.27126932
AGGGATG184200.07.2501921
ACGGGGT106800.07.20360855
AGGGAGG352900.07.19704681
Produced by FastQC (version 0.11.5)