FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005734819

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005734819
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44752115
Sequences flagged as poor quality0
Sequence length126
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT678240.15155484830158306No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA779400.064.543531
TCAACGC903850.054.6245084
CAACGCA907150.054.3728875
ATCAACG920950.053.2193683
GGTATCA357100.049.347121
ACGCAGA997500.049.2735567
AACGCAG1018400.048.5215646
TATCAAC1053200.046.9410932
CGCAGAG1084900.045.326188
GCAGAGT1252700.039.30269
AGTACGG670350.035.2013412-13
AGAGTAC1105850.034.76667410-11
TACGGGG465600.030.88170614-15
GAGTACG685050.030.084412-13
GTACGGG694500.029.70527614-15
CAGAGTA1211150.027.5258210-11
TACGGGA280050.023.32006314-15
AGTACTT719500.020.767912-13
GAGTACT620900.020.36492312-13
GTATATA290650.019.2929651
Produced by FastQC (version 0.11.5)