FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005734821

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005734821
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27054760
Sequences flagged as poor quality0
Sequence length75
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAGACG107850.012.3507921
GTCTCGA110450.010.55805333
TCGAACT81400.09.74886436
ACGGGGT89050.09.6856095
CTCGAAC80400.09.5265135
AGACGGG106850.09.5247043
TCTCGAA89600.09.47241634
CGAACTC78450.09.45580437
GAGACGG129750.09.4390822
CGGGGTT89300.09.4268126
GGTCTCG121600.09.41967232
AGAGCGA69550.09.0302931
GACGGGG101950.08.9337444
GGGTTTC272250.08.6293098
GCTGGTC260900.08.54189829
CTACTCG65550.08.5258149
CTCGATC46100.08.15760135
GTTGGCC176450.08.054708520
GTTTCAC266750.08.03116110
TCGATCT47300.07.95089336
Produced by FastQC (version 0.11.5)