FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005734822

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005734822
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27054760
Sequences flagged as poor quality0
Sequence length75
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAGACG111750.014.819311
ACGGGGT88750.012.9038145
CGGGGTT89600.012.781526
GAGACGG132000.011.838722
AGACGGG111050.011.6170923
GACGGGG104700.011.4319614
AGAGCGA71450.09.9471641
TCGAACT81100.09.74189136
CGAACTC77550.09.69852737
GTCTCGA108850.09.66668633
CTCGAAC79950.09.32099635
GGGTTTC280650.08.8252188
TCTCGAA90650.08.75333234
GGTCTCG118500.08.70480432
GTTGGCC181000.08.61522920
GTTTCAC271100.08.57654810
GCTGGTC261700.08.54217829
AGGGGCG51000.08.4561781
GCGAGAC56200.08.4085714
GAGCGAG67300.08.4063852
Produced by FastQC (version 0.11.5)