FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005734823

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005734823
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24940926
Sequences flagged as poor quality0
Sequence length75
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAGACG104500.013.1760891
ACGGGGT84000.011.4588895
GTCTCGA102000.011.02654333
AGACGGG99050.011.006043
CGGGGTT87100.010.853076
GAGACGG122900.010.8352762
GACGGGG96500.010.2962344
TCGAACT71650.09.96762836
AGAGCGA64050.09.805781
CTCGAAC73100.09.76955835
GGTCTCG111200.09.67995932
CGAACTC70950.09.43390237
TCTCGAA83450.09.09527234
CTCGATC44050.08.85022935
CTCGTGA57950.08.69203349
TCGATCT44850.08.61575236
TCTCGAT47750.08.59791134
GGGTTTC251300.08.4700998
ACCTCGT59100.08.46486247
CGTGTTA29350.08.46251317
Produced by FastQC (version 0.11.5)