FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005734824

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005734824
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24940926
Sequences flagged as poor quality0
Sequence length75
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAGACG104200.013.1110331
ACGGGGT82450.010.626345
GTCTCGA103100.010.37317233
CGGGGTT87250.010.1606076
AGGGGCG57600.010.1221711
GAGACGG124000.010.098362
AGACGGG102250.09.7828583
GACGGGG97900.09.5832524
TCGAACT75700.09.206223536
CGAACTC74500.09.03039937
GGTCTCG115150.08.92813832
AGAGCGA70250.08.8396621
GGGTTTC249600.08.8312338
CTCGAAC78900.08.70167635
TCTCGAA85850.08.47915434
TCCTGAC228700.08.29739442
AGGGACG35300.08.209451
GCTGGTC234700.08.202105529
GTTTCAC241800.08.14628610
AGAGGCG49150.08.1422411
Produced by FastQC (version 0.11.5)