FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005734825

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005734825
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26227934
Sequences flagged as poor quality0
Sequence length75
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAGACG103700.012.8119711
ACGGGGT88150.010.3322645
GTCTCGA105450.09.97858133
GAGACGG129450.09.8073822
CGGGGTT95150.09.6447486
AGACGGG104800.09.414643
AGAGCGA69300.09.11281
GACGGGG104850.09.081284
CTCGAAC78550.08.91603935
GGTCTCG119000.08.58144232
TCGAACT77350.08.38573636
TCTCGAA85850.08.35861734
CGAACTC76950.08.2948837
CGTGTTA30900.08.26124917
GGGTTTC259100.08.255188
CTCGTGA63700.08.12399949
GCTGGTC238450.08.04408229
GTTTCAC250850.07.976478610
GTTGGCC172000.07.882138320
TCGTGAT66350.07.799721750
Produced by FastQC (version 0.11.5)