FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005734826

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005734826
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26227934
Sequences flagged as poor quality0
Sequence length75
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAGACG106750.012.1523751
ACGGGGT88750.010.4162945
CGGGGTT92500.010.2923996
GAGACGG130000.09.4998582
GTCTCGA106600.09.12646233
AGACGGG108350.09.0732473
AGAGCGA72350.09.01289751
GACGGGG105500.08.8604084
AGGGACG35900.08.6494661
CGAACTC78550.08.60875437
TCGAACT80700.08.50757736
CTCGAAC79050.08.37966235
AGAGCAG265350.08.1784831
GGGTTTC265550.08.15798
GGTCTCG116950.08.14182732
TCTCGAA88750.08.12445534
GTTTCAC248350.08.05649810
AGGGGGC115450.07.88953071
GCGAGAC58050.07.6652164
AGGGGAA225900.07.57541851
Produced by FastQC (version 0.11.5)