FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005734827

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005734827
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences76518058
Sequences flagged as poor quality0
Sequence length126
%GC48

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA1036100.060.0582961
TCAACGC1201800.050.3372234
ATCAACG1220300.049.5347753
CAACGCA1233600.048.9932255
GGTATCA524250.047.2150271
AACGCAG1320950.045.921396
TATCAAC1358800.044.866132
ACGCAGA1412450.042.8569077
CGCAGAG1560600.038.78468
GCAGAGT1861200.032.7107249
AGAGTAC1536550.031.49692210-11
AGTACGG1131900.030.084112-13
TCGTATC134200.026.737999120
TACGGGG1042200.026.48277714-15
GAGTACG1188750.025.69555512-13
TGCCGTT221700.025.279089120
CAGAGTA1699450.025.02530310-11
GTACGGG1238650.024.59780914-15
CTCCGAC399000.023.27975120
ACGGGGG606350.018.99753816-17
Produced by FastQC (version 0.11.5)