FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005734828

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005734828
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences76518058
Sequences flagged as poor quality0
Sequence length126
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA841650.051.8428841
TCAACGC881750.047.3823664
ATCAACG916050.045.619463
CAACGCA930400.044.956935
AACGCAG1000600.041.8659936
TATCAAC1053050.040.4638182
ACGCAGA1051250.039.7987147
GGTATCA481700.038.334851
CGCAGAG1202500.034.7457438
AGTACGG1118250.029.78270712-13
AGAGTAC1162950.029.06576310-11
GCAGAGT1488950.028.292439
TACGGGG1034200.025.71496414-15
GAGTACG1184350.025.0089512-13
GTACGGG1235450.023.92625414-15
CAGAGTA1319950.022.71441710-11
ACGGGGG613500.018.09515416-17
GTATATA320350.017.0585441
GTATTAA334050.016.7364561
GTATTAG241800.016.0435891
Produced by FastQC (version 0.11.5)