FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005734829

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005734829
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14370551
Sequences flagged as poor quality0
Sequence length75
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAGACG51600.012.5722531
CGGGGTT41350.010.5959776
ACGGGGT40900.010.4594335
GAGACGG61200.010.09042
CGTGTTA16250.09.97741417
AGACGGG49650.09.5886843
GTCTCGA54700.09.58679833
CGAACTC36100.09.4617437
TCGATCT23350.09.45655836
GACGGGG45300.09.4433414
GGTCTCG56350.09.42856632
TCGAACT37800.09.40124636
CTCGATC22100.09.36667335
CTCGAAC37150.09.28683235
TGTCGCC26600.08.9510171
TCTCGAT25550.08.91196334
TTCACCG23900.08.8047612
TCACCGT25150.08.77858513
TCGAGCG3956.391317E-48.73441548
AGGACGA12750.08.6605371
Produced by FastQC (version 0.11.5)