FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005734830

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005734830
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14370551
Sequences flagged as poor quality0
Sequence length75
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAGACG51100.013.5715231
ACGGGGT43650.011.3798885
GAGACGG58850.010.9033752
CGGGGTT43950.010.8314346
GACGGGG47250.010.7317164
AGACGGG49250.010.5762933
GTCTCGA56650.010.29181233
CTCGAAC37800.09.9486135
CGAACTC36450.09.65454737
AGAGCGA34350.09.6426831
CTCGATC23350.09.60404535
TCGAACT38100.09.50806336
TCGATCT24150.09.00017936
GGTCTCG60750.08.97258132
CGTTGGA37000.08.85760667
GCGCTTT22550.08.72161452
AGAGCAG161700.08.7056971
TCTCGAA44150.08.6737134
AGGCGAC8404.5292836E-108.6256811
TCGTTGG37450.08.56696766
Produced by FastQC (version 0.11.5)