FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005734833

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005734833
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30552006
Sequences flagged as poor quality0
Sequence length75
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAGACG122200.011.2393621
ACGGGGT103650.09.6527525
CGGGGTT108500.09.284916
AGAGCGA79800.09.2542391
GTCTCGA125400.09.21645733
CGTATGC15800.09.17125846
AGAGCGG37000.09.0469011
GAGACGG149500.09.0229242
AGACGGG125350.08.6419573
TATGCCG15300.08.56870748
GACGGGG120550.08.528384
CTCGAAC94350.08.52000935
TCGAACT93650.08.47349236
CGAACTC89250.08.35008137
AGAGCGC30800.08.2910751
TCTCGAA104100.08.1526834
CGGCTAA62350.08.13321220
GGTCTCG141850.08.0503532
AGGGCGT29500.07.95457031
GGGTTTC323050.07.9344728
Produced by FastQC (version 0.11.5)