FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005736830

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005736830
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences849387
Sequences flagged as poor quality0
Sequence length150
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGCG700.0123.4285668
TCGGCGG1200.084.000019
TATCGGC1300.066.461537
TTATCGG1500.052.8000036
ATTATCG1801.8189894E-1244.05
TCGTCGC651.0520889E-619.93846115-19
CCGGCGA956.3955667E-918.18947430-34
CGTTACC1051.1423253E-917.82857315-19
CGATCGG652.340918E-517.723076130-134
CCAGTAC5051.3596946E-817.1089127
CGGCGAG1102.1227606E-917.0181830-34
ATCCGGC1203.7289283E-1016.80000125-29
GCGATCG602.5504155E-416.800001130-134
CAGTACC5201.9514118E-816.6153838
TCCGGCG1206.728442E-915.625-29
CGTCGCT851.3567589E-515.24705915-19
CTCGTCG902.3286098E-514.415-19
GAATCCG1405.1177267E-813.37142825-29
AGACCTC6102.1368305E-612.9836061
AATCCGG1354.7668073E-712.825-29