FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005736947

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005736947
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2345687
Sequences flagged as poor quality0
Sequence length150
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGCG4750.0112.168418
TCGGCGG5200.0102.461539
TATCGGC5450.097.7614757
TTATCGG5550.094.7027056
ATTATCG7850.066.9554145
CAGTACC16800.032.1428578
CCAGTAC17300.030.7976867
AGTACCT19950.027.4285729
GACCTCC20700.026.4347822
GCTATTA21250.024.7341182
CTCGTCG4400.024.21818215-19
TCCAGTA22950.023.8431366
AGACCTC23350.023.7430421
TCGTCGC4750.023.03999915-19
CGATCGC2350.022.6723460-64
CGTCGCT5000.021.59999815-19
GTTACCG5300.020.92075520-24
CGGCGAG5100.020.8941230-34
TCCGGCG5300.020.10566125-29
CGATCGG2750.019.89818130-134
Produced by FastQC (version 0.11.5)