FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005736969

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005736969
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2492727
Sequences flagged as poor quality0
Sequence length150
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGCG1400.066.857158
TTATCGG2350.039.829796
TCGGCGG2650.035.3207559
TATCGGC2705.456968E-1232.07
ATTATCG5451.879016E-917.1743135
TCCGGCG2302.586603E-910.64347825-29
CCGGCGA2057.0163715E-69.13170730-34
GTTACCG2353.9142553E-57.965957620-24
CGTTACC2856.778988E-67.57894715-19
CGGCGAG2702.7051557E-57.466666730-34
TCGTTAC3001.3974073E-57.200000315-19
CCGAATC2700.00164640226.420-24
TTACCGA3653.122496E-56.312329320-24
GGTTCGT3250.00205596965.7615-19
TACCGAA3050.00642292345.665573620-24
ATCCGGC3700.00160184235.44864925-29