FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005737025

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005737025
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2457211
Sequences flagged as poor quality0
Sequence length150
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGCG1350.063.9999968
TCGGCGG1750.057.69
TATCGGC2250.038.47
TTATCGG2601.200533E-1030.4615366
CCGATCG601.1753085E-519.19999955-59
TCGTCGC1453.4015102E-1014.89655215-19
ATTATCG5608.1794497E-714.1428585
CCAGTAC14500.011.9172427
CTCGTCG1851.6147897E-710.89729815-19
CGATCGC950.00812888810.61052660-64
CGTCGCT2252.0832886E-810.2415-19
TCCGGCG1707.6288597E-610.16470525-29
CAGTACC16208.758434E-98.4444458
GACCTCC18007.6033757E-108.42
CCGGCGA2056.402346E-47.726829530-34
GTTACCG2252.0514493E-47.6820-24
GCGAGAA3852.3423127E-87.480519330-34
TCCAGTA20601.0486474E-87.33980566
AGTACCT18701.11223926E-77.3155089
TCGTTAC2651.7352687E-47.06415115-19
Produced by FastQC (version 0.11.5)