FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005737166

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005737166
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences837172
Sequences flagged as poor quality0
Sequence length150
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGCG650.0121.8461468
TCGGCGG900.096.09
TTATCGG1250.069.126
TATCGGC1200.066.000017
ATTATCG2351.8189894E-1236.7659575
CGATCGG452.7998885E-522.4130-134
TCGTCGC604.876292E-721.615-19
GCGATCG555.5393975E-620.945454130-134
CGGCGAG903.270543E-919.230-34
TCCGGCG853.5497578E-818.63529425-29
CCGATCG400.00824249518.055-59
CGTCGCT857.245926E-716.94117515-19
ATCCGGC901.3163972E-616.025-29
CTCGTCG851.3567493E-515.24705915-19
TCGTTAC1201.1351585E-714.40000115-19
TACCGAA1201.1351585E-714.40000120-24
CCGGCGA902.3285935E-514.430-34
GGTTCGT1301.9343133E-814.415-19
CCAGTAC4552.5795029E-514.2417577
TCCAGTA5608.160896E-714.1428576
Produced by FastQC (version 0.11.5)