FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005737293

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005737293
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4937737
Sequences flagged as poor quality0
Sequence length150
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGCG1800.072.08
TCGGCGG3700.036.9729739
TATCGGC4300.028.4651177
TTATCGG4557.2759576E-1222.1538456
CGATCGC1856.91216E-1113.23243260-64
CCGATCG1806.2937033E-1012.855-59
ATTATCG8554.0090526E-912.6315795
TCGTCGC2301.8189894E-1212.52173915-19
CTCGTCG3103.979949E-98.82580615-19
CGTCGCT3301.3224053E-98.72727215-19
CAGTACC28105.456968E-127.1743778
CCAGTAC28908.913048E-116.72664367
CCGGCGA3654.3611017E-66.70684930-34
TCCGGCG3756.6455777E-66.52825-29
AGTACCT34006.91216E-105.92941149
AATCCGG5651.0954318E-75.86194725-29
ATCCGGC4855.8340887E-55.3443325-29
GACCTCC33951.4252691E-75.30191472
ACGGAGT17700.0056469355.28813553
TCGTTAC4958.05373E-55.236363415-19
Produced by FastQC (version 0.11.5)