FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005737314

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005737314
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5806653
Sequences flagged as poor quality0
Sequence length150
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGCG2200.052.3636368
TCGGCGG3350.034.3880589
TATCGGC4400.026.1818187
TTATCGG5306.366463E-1119.0188676
TCGTCGC2155.456968E-1212.72558115-19
CGTCGCT2857.2759576E-1210.61052615-19
CTCGTCG3104.0017767E-119.75483815-19
ATTATCG10458.6114596E-79.6459325
CCGATCG1656.444097E-48.72727255-59
TCCGGCG3102.7811875E-67.432257725-29
CGATCGC2651.735548E-47.064151360-64
CCGGCGA3451.3647454E-56.678261330-34
ACCGCGC15651.8800807E-46.44089461
GTTACCG4301.2576111E-66.362790620-24
CCAGTAC34455.822585E-95.64296057
CGTTACC5552.8781178E-65.44864915-19
CCGAATC6156.022128E-75.385365520-24
CAGTACC34151.6174636E-75.27086358
CGGCGAG4752.4394473E-45.153684630-34
TTACCGA5753.0571304E-55.008695620-24