FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005737363

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005737363
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4984802
Sequences flagged as poor quality0
Sequence length150
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGCG2500.089.280018
TATCGGC4300.051.906987
TCGGCGG4950.045.0909089
TTATCGG5600.039.8571436
ATTATCG8400.027.428575
TCGTCGC2150.017.41395415-19
CGATCGG1751.8189894E-1214.811428130-134
CTCGTCG3050.012.2754115-19
CCGATCG1451.1326338E-611.91724255-59
CGTCGCT3650.011.04657515-19
TCCGGCG4800.010.79999925-29
GTTACCG4650.010.52903220-24
CCGGCGA4900.09.991837530-34
CGGCGAG5350.09.42056130-34
CGATCGC2005.128939E-69.36000160-64
CGTTACC6050.09.28264415-19
GCGATCG2551.4385478E-79.035295130-134
TCGTTAC5200.08.58461615-19
ATCCGGC6850.07.98832125-29
TTACCGA5850.07.87692320-24
Produced by FastQC (version 0.11.5)