FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005737366

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005737366
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2462120
Sequences flagged as poor quality0
Sequence length150
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGCG954.8567017E-1060.6315778
TCGGCGG1307.821654E-944.3076939
TATCGGC2054.2954525E-728.0975597
TTATCGG2654.017633E-621.735856
ATTATCG4155.6451063E-717.3493985
CTCGTCG1301.9354047E-814.40000115-19
TCGTCGC1201.1357588E-714.40000115-19
CGATCGC800.002253668412.59999960-64
CGTCGCT1757.485869E-811.519999515-19
CGCAGAT4450.005002181511.3258435
CAGTACC13905.7734724E-99.3237428
CCAGTAC14256.768816E-67.5789477
AGTACCT16951.3638091E-67.22123869
GACCTCC16756.500294E-56.44776152
TACAGCG3303.7260592E-46.10909180-84
TCCAGTA20051.870723E-56.10473786
AGACCTC19055.8261212E-56.04724361
AGCGGTT3600.00113266945.680-84
GCGAGAA3650.00134887295.523287330-34
GTCGCTG3650.00134887295.523287320-24
Produced by FastQC (version 0.11.5)