FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005737396

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005737396
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6600023
Sequences flagged as poor quality0
Sequence length150
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGCG2200.055.6363648
TCGGCGG4450.027.5056179
TATCGGC5650.021.6637177
TTATCGG6700.019.3432836
TCGTCGC3700.010.11891915-19
ATTATCG12459.5278665E-99.8313255
CTCGTCG3853.6379788E-128.97662415-19
CGTCGCT3802.1955202E-97.95789515-19
CGATCGC2200.00135841437.260-64
CGTTACC5002.6590606E-65.766
TTACCGA6351.9900654E-75.442519720-24
TCGTTAC5307.4215295E-65.433962315-19
CCAGTAC38452.39379E-95.43042957
CAGTACC36051.677654E-85.3925118
GTTACCG5102.2577316E-55.36470620-24