FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005737397

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005737397
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6600023
Sequences flagged as poor quality0
Sequence length150
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGCG3850.050.4935078
TCGGCGG6400.031.5000029
TATCGGC7100.027.3802817
TTATCGG7900.023.6962036
ATTATCG12150.016.05
TCGTCGC3250.010.63384615-19
CTCGTCG3550.010.14084515-19
CGTCGCT4150.010.06265115-19
CGGCGAG5200.08.30769330-34
TCCGGCG4900.08.22857125-29
CCGGCGA4202.7284841E-118.22857130-34
CCGATCG2456.5790984E-57.64081655-59
GTTACCG6200.07.200000320-24
CAGTACC37053.6379788E-126.21862368
TCGTTAC6358.5128704E-106.122834715-19
CGTTACC6203.1486707E-96.0387115-19
CGATCGC2950.0044388765.85762760-64
TACCGAA9251.8189894E-125.60432520-24
CCAGTAC38955.711627E-105.54557137
TTACCGA8501.72804E-105.421176420-24
Produced by FastQC (version 0.11.5)