FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005737410

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005737410
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4942616
Sequences flagged as poor quality0
Sequence length150
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGCG2650.076.075478
TCGGCGG4400.045.8181849
TATCGGC4900.041.1428577
TTATCGG5800.038.482766
ATTATCG10400.022.1538475
CTCGTCG3500.016.8685715-19
TCGTCGC3450.016.69565215-19
CGTCGCT3450.015.02608815-19
CCGATCG1703.6779966E-912.70588355-59
CAGTACC30900.011.6504868
CCAGTAC29750.011.374797
CGATCGC2054.8785296E-911.23902560-64
CCGGCGA4000.011.16000130-34
TCCGGCG4000.010.825-29
CGATCGG1553.0724488E-510.219355130-134
GACCTCC37950.010.0553372
CGGCGAG4800.09.90000130-34
AGTACCT37100.09.7035059
TCCAGTA39400.09.6852796
ATCCGGC5700.09.34736825-29