FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005737612

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005737612
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5593120
Sequences flagged as poor quality0
Sequence length150
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGCG1650.078.5454568
TCGGCGG4050.032.09
TATCGGC4050.030.2222217
TTATCGG5050.022.811886
CTCGTCG2850.012.12631515-19
TCGTCGC2900.011.4206915-19
ATTATCG10407.23885E-810.3846165
CGTCGCT3351.8189894E-119.45671715-19
CCCGTAA6150.00508053469.3658541
TCCGGCG3504.3837645E-108.6425-29
CCGGCGA3351.7553248E-98.59701530-34
CGATCGG2056.9522415E-58.429268130-134
GCGATCG2551.2647803E-57.905883130-134
CCGATCG1850.00199939757.78378455-59
GTTACCG4105.532038E-76.673170620-24
CGTTACC4557.035305E-86.64615415-19
CGGCGAG4352.1896085E-76.620689430-34
ATCCGGC5002.6589587E-65.759999825-29
AATCCGG6201.2091368E-75.57419425-29
TCGTTAC4459.165696E-55.50112415-19
Produced by FastQC (version 0.11.5)