FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005737690

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005737690
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5668196
Sequences flagged as poor quality0
Sequence length150
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGCG3400.086.823538
TCGGCGG5400.054.6666689
TATCGGC6000.050.47
TTATCGG5850.040.6153836
ATTATCG13200.018.05
TCGTCGC3800.014.77894615-19
CGTCGCT4050.014.22222215-19
CTCGTCG4050.013.51111115-19
CCGGCGA4150.012.83855430-34
CGATCGG2201.0004442E-1011.781818130-134
TCCGGCG4100.011.59024425-29
CCGATCG1757.487506E-811.519999555-59
CAGTACC31850.011.3029828
GTTACCG5550.09.85945920-24
GCGATCG2858.731149E-109.6130-134
CCAGTAC33500.09.4567177
ATCCGGC5800.09.18620725-29
CGTTACC6650.09.09473615-19
TCGTTAC6050.08.80661215-19
CCGAATC6300.08.68571520-24