FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005737760

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005737760
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4949547
Sequences flagged as poor quality0
Sequence length150
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGCG1550.065.0322658
TCGGCGG3200.038.2499969
TATCGGC3550.028.3943677
TTATCGG4851.8189894E-1120.7835046
CCGATCG1203.4267016E-410.855-59
TCGTCGC2405.6601493E-89.615-19
ATTATCG10559.80488E-79.55450255
CTCGTCG2951.5992555E-88.78644115-19
CGATCGC1902.8300824E-48.33684360-64
CGTCGCT2703.1465115E-68.015-19
TCCGGCG3451.8021765E-67.095652625-29
TTACCGA4053.2048101E-66.420-24
CCGGCGA3703.8099206E-56.22702730-34
CGGCGAG4051.4106557E-45.68888930-34
GGTTCGT3803.6550107E-45.68421115-19
TACCGAA5051.9155903E-55.41782220-24
CAGTACC25401.2095529E-45.1023628
Produced by FastQC (version 0.11.5)