FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005737806

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005737806
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5691239
Sequences flagged as poor quality0
Sequence length150
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGCG2500.074.8800058
TATCGGC4400.042.5454567
TCGGCGG5400.034.6666689
TTATCGG5550.031.1351366
ATTATCG11600.015.5172415
TCGTCGC3250.014.1784615-19
CTCGTCG3600.013.615-19
CGTCGCT3750.011.90399915-19
CGATCGG1603.699046E-610.8130-134
CCGATCG1553.450814E-49.29032255-59
TCCGGCG4400.08.83636425-29
GCGATCG1801.5763735E-48.8130-134
CCAGTAC31100.08.3344057
CAGTACC33400.07.7604798
CCGGCGA4858.185452E-117.422680430-34
CGATCGC1950.00334964677.384615460-64
CGTTACC5451.6007107E-106.86972515-19
ATCCGGC6550.06.81526725-29
CGGCGAG5603.0013325E-106.685714230-34
GTTACCG4156.8219924E-76.592770620-24
Produced by FastQC (version 0.11.5)