FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005737828

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005737828
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1620459
Sequences flagged as poor quality0
Sequence length150
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGCG950.075.7894748
TCGGCGG1352.0372681E-1048.09
TATCGGC1557.930794E-1041.806457
TTATCGG1959.242156E-625.8461556
ATTATCG2400.001110490518.05
TCGTCGC708.2333805E-414.40000115-19
CTCGTCG953.8758346E-513.64210515-19
CGTCGCT750.001386622213.44000115-19
ACCGCGC3900.001861965512.9230781
TCCGGCG1254.994377E-410.36825-29
CCGGCGA1550.0035570918.3612930-34
GCGGGTT1902.829469E-48.33684310-14
CGGCGAG2056.401656E-47.726829530-34
CGAATCC2056.401656E-47.726829525-29
CAGTACC8550.0085882337.57894758
GAATCCG2455.460876E-47.05306125-29
GGGTTCG2100.0068847846.857143410-14
GCGAGAA2650.00133295096.52075530-34
AAACCGC3253.0600297E-46.203077105-109
AACCGCT3100.00116798536.038709105-109
Produced by FastQC (version 0.11.5)