FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005737874

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005737874
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2367201
Sequences flagged as poor quality0
Sequence length150
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGACCTCCAGTACCTCCTCGTCGCTGGTATAACCTTCACCGTAAAAGCTC108660.45902312477901114No Hit
TGCTATTATCGGCGGGTTCGTTACCGAATCCGGCGAGAAAATTAAGGGTC77550.32760209209103913No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGCG20200.0135.445548
TCGGCGG20600.0133.165049
TATCGGC21050.0129.976247
TTATCGG19500.0128.49236
ATTATCG21050.0119.7149665
CCAGTAC32150.087.5645457
CAGTACC33400.083.8562858
AGTACCT35050.079.90879
GACCTCC35500.079.504232
TCCAGTA37300.076.246646
AGACCTC37750.075.1470261
GCTATTA36550.069.340632
CTCCAGT46450.061.072125
TATTATC44600.056.502244
ACCTCCA50350.056.341613
CTATTAT47150.053.599153
ATATCGG2750.052.3636366
TGCTATT49750.050.7979931
CCTCCAG56000.050.7857134
CTATCGG2009.587893E-932.3999986
Produced by FastQC (version 0.11.5)