FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005737880

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005737880
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1627186
Sequences flagged as poor quality0
Sequence length150
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGCG1050.082.285728
TCGGCGG1300.066.461549
TATCGGC1450.059.586217
TTATCGG1700.046.5882346
ATTATCG4052.0399966E-819.5555555
TCGTCGC704.4219734E-516.45714415-19
CGTCGCT902.329127E-516.08
GCGATCG708.233385E-414.400001130-134
CTCGTCG902.329127E-514.415-19
TCCGGCG1251.8696483E-713.82399925-29
CCGGCGA1201.7802158E-613.230-34
CGTTACC1502.1396077E-510.5615-19
ATCCGGC2055.8520527E-810.53658525-29
CGGCGAG1553.0717565E-510.21935630-34
TCGTTAC1553.0717565E-510.21935615-19
AATCCGG2005.127373E-69.36000125-29
GGGTTCG2252.3482644E-68.9610-14
CCAGTAC9703.635345E-58.9072177
GTTACCG1656.44284E-48.7272736
CCGAATC2151.2109194E-48.037209520-24
Produced by FastQC (version 0.11.5)