FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005737896

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005737896
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2435020
Sequences flagged as poor quality0
Sequence length150
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGCG950.0106.105268
TTATCGG1950.055.3846136
TATCGGC1950.055.3846137
TCGGCGG2350.042.8936169
ATTATCG4751.2732926E-1121.2210525
TCGTCGC1150.020.03478215-19
CCGATCG902.3293143E-514.455-59
CGATCGC1059.903566E-512.34285660-64
CTCGTCG2002.5465852E-1012.2415-19
CCGGCGA1902.6672733E-611.3684213
CGGCGAG1654.3478576E-711.34545430-34
GCGATCG900.005438579211.2130-134
CGTCGCT1952.8063369E-811.07692215-19
CGATCGG1050.001367774110.971428130-134
CCAGTAC12551.0842996E-89.7529897
TCCGGCG1708.459818E-59.31764725-29
AGTACCT17957.8216544E-118.82451259
TCCAGTA17303.4742698E-108.7398846
CAGTACC14551.1952397E-78.4123728
AGCGGTT3402.0721927E-88.04705980-84
Produced by FastQC (version 0.11.5)