FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005737901

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005737901
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1622433
Sequences flagged as poor quality0
Sequence length150
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGCG1600.085.58
TCGGCGG1650.082.9090969
TATCGGC2000.072.000017
TTATCGG2300.050.0869566
ATTATCG3450.033.3913045
CGATCGG801.7713319E-819.8130-134
TCGTCGC1600.018.915-19
CTCGTCG1550.018.58064715-19
CAGTACC9550.017.3403138
TCCGGCG1650.016.5818225-29
CCAGTAC9850.016.0812197
CCGATCG901.3167701E-616.055-59
CCGGCGA1750.015.63428630-34
CGTCGCT1950.014.03076815-19
ACCGCGC3250.00836794813.2923081
AGTACCT13600.013.2352949
CGGCGAG2351.8189894E-1212.25531930-34
GTTACCG2201.0004442E-1011.78181820-24
GACCTCC12951.8189894E-1211.6756752
GGTTCGT2251.4733814E-1011.5215-19
Produced by FastQC (version 0.11.5)