FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005738381

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005738381
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3294560
Sequences flagged as poor quality0
Sequence length150
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGCG2200.094.909088
TCGGCGG3550.058.8169029
TATCGGC3550.058.8169027
TTATCGG3550.054.7605636
ATTATCG6050.033.3223155
GCGATCG1750.018.102858130-134
CGATCGG1900.016.673683130-134
CTCGTCG2350.015.31914915-19
TCGTCGC2200.015.05454515-19
ACCGCGC9100.015.0329671
CCGGCGA2700.014.93333330-34
TCCGGCG2950.013.66779625-29
CGGCGAG3250.013.29230830-34
GTTACCG3150.013.25714320-24
GCGCGAT5459.595462E-613.211012
CCGATCG1354.7695903E-712.855-59
CGCGATC5851.9880263E-512.3076933
CGTCGCT3000.012.015-19
GGCGCGA6455.3936852E-511.162791
TACCGAA4450.010.67865220-24
Produced by FastQC (version 0.11.5)