FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005738448

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005738448
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2466141
Sequences flagged as poor quality0
Sequence length150
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGCG900.080.08
TCGGCGG1752.6029738E-937.0285729
TATCGGC1851.7482307E-731.1351367
TTATCGG2301.1728553E-625.0434786
CGCGATC3859.978078E-514.961043
CGTCGCT1609.640644E-1114.40000115-19
CTCGTCG1855.456968E-1214.01081115-19
TCGTCGC1651.5825208E-1013.96363515-19
CGCAATC4252.3110282E-413.5529413
GCGCAAT4302.551657E-413.39534952
CCGATCG903.7492596E-412.855-59
ATTATCG4503.747365E-412.85
ACCGCGC6052.8049015E-511.9008271
CCAGTAC13251.8189894E-1211.4113217
GCGCGAT4800.00878186610.5000012
CGATCGC1100.002015445810.47272760-64
CAGTACC13804.3655746E-1110.4347838
AGACCTC16650.010.3783781
TCCGGCG1707.628867E-610.16470525-29
CGATCTT5950.00386641979.6806735
Produced by FastQC (version 0.11.5)