FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005738566

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005738566
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2482908
Sequences flagged as poor quality0
Sequence length150
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGACCTCCAGTACCTCCTCGTCGCTGGTATAACCTTCACCGTAAAAGCTC107750.4339669452110187No Hit
TGCTATTATCGGCGGGTTCGTTACCGAATCCGGCGAGAAAATTAAGGGTC61400.24729067689982873No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGCG18000.0134.88
TATCGGC18400.0131.869577
TTATCGG18200.0131.736256
TCGGCGG18800.0129.063839
ATTATCG20850.0114.9928055
CCAGTAC33650.089.01047
CAGTACC33950.088.648018
GACCTCC37650.079.553792
AGTACCT37750.079.343059
TCCAGTA37950.079.1146246
AGACCTC40400.073.782181
GCTATTA34450.069.387522
CTCCAGT46650.064.668815
TATTATC45150.053.4219254
ACCTCCA56550.053.2201583
TGCTATT48600.049.1851841
CTATTAT49850.048.385153
CCTCCAG63750.047.435294
TCGTCGC20800.028.10769315-19
CGTCGCT21000.027.56571615-19
Produced by FastQC (version 0.11.5)