FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005738567

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005738567
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2482908
Sequences flagged as poor quality0
Sequence length150
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGACCTCCAGTACCTCCTCGTCGCTGGTATAACCTTCACCGTAAAAGCTC105350.42430086012047163No Hit
TGCTATTATCGGCGGGTTCGTTACCGAATCCGGCGAGAAAATTAAGGGTC74850.3014610287614362No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGCG20400.0138.705898
TCGGCGG21150.0134.127669
TATCGGC21250.0133.496487
TTATCGG22100.0128.03626
ATTATCG23400.0121.230775
CAGTACC29950.086.5442358
CCAGTAC33050.077.555227
AGTACCT33150.077.538479
AGACCTC35400.073.0169451
GCTATTA39700.072.02
GACCTCC35950.071.699582
TCCAGTA38300.067.488256
TATTATC50750.057.174394
CTCCAGT45550.056.746435
TGCTATT50700.055.668641
CTATTAT54850.052.2443053
ACCTCCA50600.050.6561283
CCTCCAG57500.044.9530454
TCGTCGC18450.027.47317115-19
TCCGGCG20550.027.18832225-29
Produced by FastQC (version 0.11.5)