FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005738625

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005738625
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2472046
Sequences flagged as poor quality0
Sequence length150
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGCG1600.099.08
TCGGCGG2100.075.4285669
TATCGGC3150.050.2857177
TTATCGG3150.048.0000046
ATTATCG4100.036.878055
TCGTCGC1202.0008883E-1118.015-19
CCGATCG500.00146078217.27999955-59
CTCGTCG1306.548362E-1116.61538515-19
CGTCGCT1559.240466E-1013.93548315-19
GCGATCG1303.0151932E-713.292308130-134
TCCGGCG2250.012.825-29
GTTACCG2850.012.63157920-24
GCGCGAT4100.002711346112.2926842
ATCCGGC2750.011.5225-29
CGATCGG1409.965539E-611.314285130-134
CCGGCGA2559.094947E-1211.29411730-34
TCGTTAC2950.011.227118515-19
GGCGCGA4550.005902683411.0769231
CGTTACC3150.010.51428615-19
GGTTCGT3300.010.47272815-19
Produced by FastQC (version 0.11.5)