FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005738682

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005738682
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3310011
Sequences flagged as poor quality0
Sequence length150
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGCG2100.078.857148
TCGGCGG2450.064.653069
TATCGGC3950.041.924057
TTATCGG3700.040.8648646
ATTATCG6750.022.45
TCGTCGC2150.018.7534915-19
CTCGTCG2350.017.77021215-19
CGTCGCT2600.016.06153915-19
CCGGCGA2301.8189894E-1212.52173930-34
TCCGGCG2403.6379788E-1211.99999925-29
CAGTACC18750.011.9039998
CCAGTAC18450.011.7073177
ACCGCGC9351.5106707E-811.5508011
CCGATCG1654.3482214E-711.34545455-59
CGATCGG1305.8381127E-511.076923130-134
CGTTACC3453.6379788E-129.615-19
TTACCGA3800.09.47368420-24
GCGATCG1553.4506182E-49.290323130-134
AGTACCT23450.09.2110879
TCGTTAC3950.09.11392515-19