FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005738723

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005738723
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3275461
Sequences flagged as poor quality0
Sequence length150
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGCG2050.070.2439048
TCGGCGG2650.054.3396229
TATCGGC2850.048.07
TTATCGG3700.035.0270276
ATTATCG6100.021.2459015
TCGTCGC2050.017.56097615-19
CCGATCG1251.1545126E-814.97600155-59
CGATCGG1555.820766E-1114.864515130-134
TCCGGCG2600.014.40000125-29
CTCGTCG2650.014.12830215-19
CGTCGCT2600.013.84615415-19
GCGATCG1907.2759576E-1213.642105130-134
CCGGCGA3250.010.63384630-34
GTTACCG3250.010.63384620-24
CGATCGC1902.3324901E-710.61052660-64
CGGCGAG3450.010.43478330-34
CAGTACC20050.09.695768
ATCCGGC4350.09.625-29
CCAGTAC19900.09.4070367
TTACCGA3752.0008883E-118.83220-24